The Department of Anesthesiology, Perioperative and Pain Medicine at Stanford University is seeking a Life Science Research Professional 2 (1-year Fixed Term), to perform complex functions and activities involved in defined research projects, and independently conduct a portion of the research project(s).

The Peltz lab has received NIH funding for two projects that develop an AI pipeline for mouse genetic discovery. For these projects, we must generate and characterize knockin mice with reversions of disease-causing mutations for several diseases. As a consequence, we have an opening for a 1-year fixed term LSRP2 position, for a person with extensive experience (>10 years) in mouse reproduction (superovulation, embryo preparation, etc.), mouse colony maintenance; and with mouse genome engineering methods that utilize embryo or stem cell microinjection, and with CRISPR and other DNA recombination methods that are used to generate knockin mice.This individual will also be responsible for maintaining all supplies (bedding, food, etc.) for mouse colony maintenance, and genotyping supplies.

At Stanford University School of Medicine, the work we do touches the lives of those today and tomorrow. Through education, research, and healthcare, the School of Medicine improves health through leadership, diversity, collaborative discoveries, and innovation in health care. Whether working in departments with faculty, or in units that support the school, our staff are a part of teams that propel us on our journey toward the future of medicine and precision health.

Stanford is rooted in a culture of excellence and values innovation, collaboration, and life-long learning. To foster the talents and aspirations of our staff, Stanford offers career development programs, competitive pay that reflects market trends and benefits that increase financial stability and promote healthy, fulfilling lives. An award- winning employer, Stanford offers an exceptional setting for professionals looking to advance their careers.

The School of Medicine, and the Department of Anesthesia are committed to diversity, equity, and inclusion for its faculty, staff, residents, postdocs, and fellows. We aim to recruit, support, retain, and promote diversity in our department.

For more information on our department, please see our website: https://med.stanford.edu/anesthesia.html.

Duties include:

• Generate Genome engineered mice. This requires super-ovulating mice, generating fertilized embryos, injecting gRNAs and rDNA into the embryos, re-implantation of embryos into pseudo-pregnant mice. This requires independently design the gRNAs that are used for CRISPR engineering of the mouse genome, adjust the dosage of CRISPR enzyme and alter the cell permeation methods used for genome engineering, and altering the superovulation protocols for the different strains whose genomes will be engineered.
• Responsible for independently designing the strategy for generation of each of the genome engineered mouse lines.
• Care and maintenance of the colonies of genome engineered mice. This requires monitoring the health of the mice, genotyping the mice at weaning, establishing the breeding pairs to generating the lines, and maintaining the colonies of pseudo-pregnant females.
• Determine if the mice can be generated by CRISPR engineering methods or if conventional non-homologous end joining methods can be used. This is performed along and all of other colony maintenance responsibilities.
• Determine if mice have the appropriate genome modifications, modify protocols for super-ovulation of the different strains whose genomes will be engineered.
• Develop new protocols for genome engineering of inbred strains, which have disease phenotypes, but are not commonly used for genome engineering.
• Report results to the two PIs (Stankowitz and Peltz) for the hearing project, and to Peltz lab personnel for the R24 project.
• This person will keep abreast of new technical developments and implement them in his/her work.
• Will write sections of research papers describing how genome engineered mice are prepared and characterized.
• The individual will be responsible for ordering the strains of mice that are needed, maintain the supplies (food, bedding, etc.) required for colony maintenance, genotyping supplies.
• Will be responsible for the regular APLAAC inspections and that we conform with our APLAAC protocol.
• Will assist in the supervision of post-doctoral fellows that are working on the project.
• Will be responsible for compliance with APPLAC protocols, and the appropriate procedures followed.

*- Other duties may also be assigned